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Docosahexaenoic acid, or DHA, is an essential polyunsaturated fatty acid for human body. It is a straight chain fatty acid containing 22 carbon atoms and 6 double bonds, which is abundant in fish oil. Animal glycerophosphingolipids contain different amounts of DHA, which can be α- Linolenic acid is produced, but the amount is low, and it is mainly supplemented by food.
1. Docosahexaenoic acid can assist the development of brain cells, help to enhance learning and memory function, help the healthy development of brain and nerve, help to prevent vision loss, and help to prevent and treat Alzheimer's disease.
2. DHA has antioxidant and anti-aging effects.
3. DHA can reduce serum total cholesterol and low-density lipoprotein cholesterol, increase high-density lipoprotein cholesterol, and treat hyperlipidemia and atherosclerosis.
1. Low temperature classification method
The solubility differences of different fatty acids in supercooled organic solvents were used to separate and concentrate DHA. Dissolve the fish oil in 1 to 10 times anhydrous acetone and cool to below - 25 ℃. The lower layer of the mixed liquid forms crystals containing a large amount of saturated fatty acids and low degree unsaturated fatty acids, while the upper layer contains a large amount of highly unsaturated fatty acids in acetone solution. A fish oil preparation with a high DHA content can be obtained by filtering the mixture and distilling the filtrate under vacuum to remove acetone. To improve the separation effect, a small amount of hydrophilic solvent such as water or alcohol can be added to anhydrous acetone.
2. Solvent extraction method
DHA is separated and concentrated using metal salts of different fatty acids and differences in solubility in certain organic solvents. Mix ethanol, fish oil, and NaOH in a certain proportion, and then heat to saponify the fish oil. The saponified mixed liquid is subjected to pressure filtration to obtain soap liquid and soap particles, respectively. Add H2SO4 to the soap solution under stirring until the pH is 1-2. Separate the upper layer of crude fatty acid ethanol mixture, heat to recover ethanol, and repeatedly wash the fatty acid to neutral to obtain a refined fish oil with a high DHA content.
3. Fungal fermentation
The research on the production of DHA by fungal fermentation mainly focuses on Chrysanthemum capsulatum and Chrysanthemum schizogenum, both of which are marine fungi with pigment and light stimulating growth characteristics. Using fungal fermentation to produce DHA can overcome the shortage of obtaining DHA from fish oil, manually control the influencing factors, and maintain the stability of DHA production and content. When fungi ferment to produce DHA, they generally synthesize less EPA and other polyunsaturated fatty acids, which is conducive to the separation and concentration of DHA and the preparation of high-purity DHA.
Docosahexaenoic acid, or DHA, is an essential polyunsaturated fatty acid for human body. It is a straight chain fatty acid containing 22 carbon atoms and 6 double bonds, which is abundant in fish oil. Animal glycerophosphingolipids contain different amounts of DHA, which can be α- Linolenic acid is produced, but the amount is low, and it is mainly supplemented by food.
1. Docosahexaenoic acid can assist the development of brain cells, help to enhance learning and memory function, help the healthy development of brain and nerve, help to prevent vision loss, and help to prevent and treat Alzheimer's disease.
2. DHA has antioxidant and anti-aging effects.
3. DHA can reduce serum total cholesterol and low-density lipoprotein cholesterol, increase high-density lipoprotein cholesterol, and treat hyperlipidemia and atherosclerosis.
1. Low temperature classification method
The solubility differences of different fatty acids in supercooled organic solvents were used to separate and concentrate DHA. Dissolve the fish oil in 1 to 10 times anhydrous acetone and cool to below - 25 ℃. The lower layer of the mixed liquid forms crystals containing a large amount of saturated fatty acids and low degree unsaturated fatty acids, while the upper layer contains a large amount of highly unsaturated fatty acids in acetone solution. A fish oil preparation with a high DHA content can be obtained by filtering the mixture and distilling the filtrate under vacuum to remove acetone. To improve the separation effect, a small amount of hydrophilic solvent such as water or alcohol can be added to anhydrous acetone.
2. Solvent extraction method
DHA is separated and concentrated using metal salts of different fatty acids and differences in solubility in certain organic solvents. Mix ethanol, fish oil, and NaOH in a certain proportion, and then heat to saponify the fish oil. The saponified mixed liquid is subjected to pressure filtration to obtain soap liquid and soap particles, respectively. Add H2SO4 to the soap solution under stirring until the pH is 1-2. Separate the upper layer of crude fatty acid ethanol mixture, heat to recover ethanol, and repeatedly wash the fatty acid to neutral to obtain a refined fish oil with a high DHA content.
3. Fungal fermentation
The research on the production of DHA by fungal fermentation mainly focuses on Chrysanthemum capsulatum and Chrysanthemum schizogenum, both of which are marine fungi with pigment and light stimulating growth characteristics. Using fungal fermentation to produce DHA can overcome the shortage of obtaining DHA from fish oil, manually control the influencing factors, and maintain the stability of DHA production and content. When fungi ferment to produce DHA, they generally synthesize less EPA and other polyunsaturated fatty acids, which is conducive to the separation and concentration of DHA and the preparation of high-purity DHA.
