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Honokiol is a white powder, easily soluble in benzene, ether, chloroform, ethanol, etc., but hardly soluble in water. It reacts with methanol solution of ferric trichloride to show blue-black color, reacts with Millon reagent to produce brown precipitate, and reacts with phloroglucinol hydrochloric acid solution to produce red precipitate.
1. Production process of Magnolia officinalis extract (fluid extract)
Take magnolia bark and crush it, infiltrate it with ethanol for 12 hours, place it in a percolation cylinder, use about 12 times the amount of ethanol for percolation, collect the percolation liquid, recover the ethanol under reduced pressure to the end, and obtain a liquid extract. The yield is about 9%, the content of solids is 85.0%, containing more than 11.0% of magnolol and more than 5.0% of honokiol.
2. Extraction and separation of magnolol and honokiol
Take Magnolia officinalis dry skin coarse powder, add 1/5 amount (W/W) of quicklime powder, after mixing well, use 15-20 times the amount of distilled water to infiltrate, add hydrochloric acid to the percolation solution to adjust the pH value to 2-3, stand still. Collect the precipitated precipitate, wash it with distilled water until the pH value of the precipitate is 6-7, add alumina (1:10) after drying, mix well, put it in an extractor, and extract it with cyclohexane. Concentrate the cyclohexane extract, let it cool, and precipitate white crystals, and filter to obtain the crystals and mother liquor. Crystallization with cyclohexane recrystallization, namely honokiol. After the mother liquor is concentrated, crystals are precipitated, and cyclohexane recrystallizes to obtain colorless flaky crystals, which are magnolol crystals. The yield of magnolol was 85%, and that of honokiol was 74%.
1. Role of disease resistant microorganisms
Magnolia officinalis decoction has inhibitory effect on gram-positive bacteria such as staphylococcus, hemolytic streptococcus, pneumococcus, pertussis bacillus, and gram-negative bacteria such as anthrax bacillus, dysentery bacillus, typhoid bacillus, paratyphoid bacillus, vibrio cholerae, escherichia coli, proteus bacillus, bacillus subtilis, diphtheria, among which staphylococcus has the strongest inhibitory effect. At the concentration of 15%, it can inhibit the skin fungi of experimental animals, such as Tinea minor, Trichophyton concentric, Trichophyton rubrum, and Trichophyton aescinatum. The decoction of Magnolia officinalis has some effects on improving the substantial pathological damage of experimental viral hepatitis in mice.
Magnolol has significant antibacterial activity against gram-positive bacteria and acid resistant bacteria; The anti caries effect of magnolol is very significant, and the minimum inhibitory concentration is 6.3 μ G/ml, its antibacterial activity is stronger than the typical antibacterial alkaloid berberine (MIC: 50 μ g/ml)。
The saturated aqueous solution of Magnolia officinalis and Magnolia officinalis volatile oil has a certain antibacterial effect on Staphylococcus aureus, Sarcina and Bacillus subtilis.
2. Effect on cardiovascular system
Magnolol and honokiol inhibit the formation of thromboxane B2 under various conditions, and the increase of intracellular Ca2+caused by arachidonic acid or collagen is also inhibited by both.
Honokiol can inhibit the activity of cyclic nucleotide phosphodiesterase stimulated by CaM, and Honokiol can combine with CaM in the presence of Ca2+, thus antagonizing its activation of phosphodiesterase. In addition, honokiol can stimulate the basic activity of CaM dependent phosphodiesterase.
3. Anti tumor effect
Magnolol and its hydroxymethyl derivatives have obvious inhibitory effect on the second stage skin tumor in mice. Lignans, magnolol, magnolol, magnolol and monoterpene magnolol are antagonists of the activation of Epstein Barr virus early antigen (EBv EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA).
The methanol extract of Magnolia officinalis and magnolol have obvious inhibitory effect on the skin tumor of mice caused by the second stage carcinogenesis experiment in vivo.
Honokiol is a white powder, easily soluble in benzene, ether, chloroform, ethanol, etc., but hardly soluble in water. It reacts with methanol solution of ferric trichloride to show blue-black color, reacts with Millon reagent to produce brown precipitate, and reacts with phloroglucinol hydrochloric acid solution to produce red precipitate.
1. Production process of Magnolia officinalis extract (fluid extract)
Take magnolia bark and crush it, infiltrate it with ethanol for 12 hours, place it in a percolation cylinder, use about 12 times the amount of ethanol for percolation, collect the percolation liquid, recover the ethanol under reduced pressure to the end, and obtain a liquid extract. The yield is about 9%, the content of solids is 85.0%, containing more than 11.0% of magnolol and more than 5.0% of honokiol.
2. Extraction and separation of magnolol and honokiol
Take Magnolia officinalis dry skin coarse powder, add 1/5 amount (W/W) of quicklime powder, after mixing well, use 15-20 times the amount of distilled water to infiltrate, add hydrochloric acid to the percolation solution to adjust the pH value to 2-3, stand still. Collect the precipitated precipitate, wash it with distilled water until the pH value of the precipitate is 6-7, add alumina (1:10) after drying, mix well, put it in an extractor, and extract it with cyclohexane. Concentrate the cyclohexane extract, let it cool, and precipitate white crystals, and filter to obtain the crystals and mother liquor. Crystallization with cyclohexane recrystallization, namely honokiol. After the mother liquor is concentrated, crystals are precipitated, and cyclohexane recrystallizes to obtain colorless flaky crystals, which are magnolol crystals. The yield of magnolol was 85%, and that of honokiol was 74%.
1. Role of disease resistant microorganisms
Magnolia officinalis decoction has inhibitory effect on gram-positive bacteria such as staphylococcus, hemolytic streptococcus, pneumococcus, pertussis bacillus, and gram-negative bacteria such as anthrax bacillus, dysentery bacillus, typhoid bacillus, paratyphoid bacillus, vibrio cholerae, escherichia coli, proteus bacillus, bacillus subtilis, diphtheria, among which staphylococcus has the strongest inhibitory effect. At the concentration of 15%, it can inhibit the skin fungi of experimental animals, such as Tinea minor, Trichophyton concentric, Trichophyton rubrum, and Trichophyton aescinatum. The decoction of Magnolia officinalis has some effects on improving the substantial pathological damage of experimental viral hepatitis in mice.
Magnolol has significant antibacterial activity against gram-positive bacteria and acid resistant bacteria; The anti caries effect of magnolol is very significant, and the minimum inhibitory concentration is 6.3 μ G/ml, its antibacterial activity is stronger than the typical antibacterial alkaloid berberine (MIC: 50 μ g/ml)。
The saturated aqueous solution of Magnolia officinalis and Magnolia officinalis volatile oil has a certain antibacterial effect on Staphylococcus aureus, Sarcina and Bacillus subtilis.
2. Effect on cardiovascular system
Magnolol and honokiol inhibit the formation of thromboxane B2 under various conditions, and the increase of intracellular Ca2+caused by arachidonic acid or collagen is also inhibited by both.
Honokiol can inhibit the activity of cyclic nucleotide phosphodiesterase stimulated by CaM, and Honokiol can combine with CaM in the presence of Ca2+, thus antagonizing its activation of phosphodiesterase. In addition, honokiol can stimulate the basic activity of CaM dependent phosphodiesterase.
3. Anti tumor effect
Magnolol and its hydroxymethyl derivatives have obvious inhibitory effect on the second stage skin tumor in mice. Lignans, magnolol, magnolol, magnolol and monoterpene magnolol are antagonists of the activation of Epstein Barr virus early antigen (EBv EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA).
The methanol extract of Magnolia officinalis and magnolol have obvious inhibitory effect on the skin tumor of mice caused by the second stage carcinogenesis experiment in vivo.
