Hyodesoxycholic Acid
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Hyodesoxycholic Acid

Product Name:Hyodesoxycholic Acid
CAS NO.:83-49-8
Molecular formula:C24H40O4
Molecular weight:392.58
Appearance:White or slightly yellowish powder
EINECS NO.: 201-483-2

Hyodesoxycholic acid introduction

Hyodeoxycholic acid is an organic compound. It is a kind of cholic acid extracted from pig bile. It is white or slightly yellow powder and tastes bitter. It smells slightly fishy. Slightly soluble in alcohol, slightly soluble in acetone, very slightly soluble in ether and chloroform, and almost insoluble in water.

It can inhibit the formation of cholic acid and dissolve fat, reduce cholesterol and triglyceride in blood, and is applicable to type Ia or type Ib hyperlipidemia, atherosclerosis, and has a certain antibacterial effect on pertussis, diphtheria, staphylococcus aureus, etc. It can also be used as anti-inflammatory drugs to treat chronic bronchitis, viral upper respiratory tract inflammation in children, etc.

Hyodesoxycholic acid function and purpose

This product can stimulate the secretion of bile, make the bile thinner without increasing the solid volume, and is suitable for cholangitis, cholecystitis, cholelithiasis and other non-obstructive cholestasis with adenosine methionine succinate; It can also accelerate the excretion of gallbladder contrast agent from the liver and help to develop. It can also promote intestinal fat decomposition and absorption of fat-soluble vitamins, and can be used for indigestion caused by liver and gallbladder diseases, reduce blood cholesterol, and treat and prevent coronary heart disease, hypertension, etc.

Hyodesoxycholic acid production method

Fresh pig bile as raw material

Preparation of pig bile acid: take fresh pig bile, add the supernatant of saturated limewater and heat it to boiling to prepare calcium bilirubin salt. The mother liquor is acidified with hydrochloric acid while it is hot until Congo red turns blue, precipitate the black colloidal precipitate combined with pig bile acid, remove the emulsion, collect the precipitate, and wash it with tap water to become hard and brittle to obtain pig bile acid.

Pig bile [saturated limewater] → [pH11-12, 100 ℃] mother liquor [HCl] → sediment [water] → pig bile acid

Preparation of crude product: take crude pig bile acid, add 1.5 times the mass of sodium hydroxide, add 9 times the volume of water, heat for more than 16 hours, cool, stand and layer, pour out the upper layer of light yellow liquid, dissolve the precipitate with a small amount of water, add dilute hydrochloric acid or sulfuric acid (2:1) to acidize until the Congo red test paper turns blue, take out the precipitate, filter, wash it to neutral, golden, vacuum dry, and get the crude product.

Pig bile acid [NaOH, water] → [>16h] saponification solution [HCl] → crude product

Preparation of the finished product of isodeoxycholic acid: take the crude product, add 5 times the amount of ethyl acetate, add 150-200g/L activated carbon, heat, stir, reflow, dissolve, cool, filter, add 3 times the amount of ethyl acetate to reflow, filter, combine the filtrate twice, add 200g/L anhydrous sodium sulfate, and filter to obtain the filtrate. Concentrate to 1/5-1/3 of the original volume, cool, precipitate crystals, filter, obtain crystals, wash with ethyl acetate, and vacuum dry to obtain the finished product of isodeoxycholic acid.

Crude product [ethyl acetate, activated carbon] → filtrate [anhydrous Na2SO4] → filtrate → crystal [ethyl acetate] → finished product of isodeoxycholic acid.